Home
/CNC
|
Biotechnology Home |
Faculty |
Resources |
Application |
Planning Sheets |
Courses/Syllabi
 SOLUTION AND MEDIA PREPARATION
(BioSc 245, summer quarter)
This is a laboratory course in the preparation of
solutions and media commonly used in the biotechnology
industry. Media typically used in biotechnology and
microbiology laboratories will be prepared according to
protocols, autoclaved and poured for use. Below is a
list of some of the
laboratory procedures that will be performed:
- Preparation of assigned media and solutions
- Calculations required for solution and media preparation
- Use of Mettler scales, pH meters, pipettors, centrifuges
and stir plates
- Autoclave, centrifuge and filter sterilization
- Storage considerations
- Standard laboratory operating and safety procedures
- Hazard categories, MSDS and disposal procedures
- FDA quality control requirements, documentation,
reproducibility
-
Download the course syllabus
 MOLECULAR BIOLOGY
(BioSc 250/251, fall quarter)
This course examines the molecular structures and
processes that underlie cellular function. Students
completing the course will have the fundamental
knowledge necessary to understand current research in
molecular biology. The following laboratory procedures
will be performed in the lab portion of the class:
- Aseptic techniques for bacterial transfers
- Use of pH meters and spectrophotometers
- Extraction and quantification of chromosomal DNA using
UV absorbance and ethidium bromide
- Restriction digestion of lambda phage DNA and agarose
gel electrophoresis
- Transformation of E. coli with plasmids
- Protein assay: Lowry Method
- Enzyme assays
- Sephadex column chromatography
- Ammonium sulfate protein purification
- Protein gel electrophoresis: SDS-polyacrylamide gel
- Western blots for isolation of specific proteins
-
Download the course syllabus
 RECOMBINANT DNA
(BioSc 265, winter quarter)
This basic course explores the theory and concepts of
recombinant DNA techniques. The following laboratory
procedures will be performed in the lab portion of the
class:
- Growth and maintenance of E. coli and lambda phage
- Plaque assays for viral titers
- Isolation and gel analysis of plasmid DNA
- Preparation and analysis of vectors, ligation>
- Transformation with pLUX2 and pUC19 genes
- Restriction analysis of recombinant plasmids
- DNA sequencing and use of GenBank to compare with known
sequences
- Southern blots for isolation of specific DNA fragments
- Polymerase chain reaction and gel analysis of products
- Polaroid photography of gels
- Internet and World Wide Web searches
-
Download the course syllabus
 IMMUNOLOGY
(BioSc 270, spring quarter)
This course focuses on the concepts necessary to
understand basic immunology, including antigen and
antibody structure and function, the genetic basis of
antibody production, humoral and cellular based
immunity, role of the major histocompatibility complex,
control mechanisms, autoimmunity, innate and
acquired immunity. The following laboratory
procedures will be performed:
- Quantitative immunoassays such as dot blots
- Immunoaffinity columns for protein purification
- Enzyme-linked immunosorbent assays (ELISA)
- Antigen isolation techniques using primary and secondary
antibodies
- Monoclonal antibody techniques
-
Download the course syllabus
 BIOTECHNOLOGY TECHNIQUES
(BioSc 275, spring quarter)
This laboratory course will focus on the techniques
currently prevalent in the manufacturing and
production aspect of the biotechnology industry. The
main focus of the course is on the isolation and
purification of proteins. The following laboratory
procedures will be performed in the lab portion of the
class:
- Cation-anion exchange chromatography
- Protein isolation using hydrophobic interactions
- High performance liquid chromatography (HPLC) for
protein purification
- Ultrafiltration for protein concentration
- Isoelectric focusing
- Exposure to good manufacturing practices (GMP), quality
control
- FDA regulations, clinical trials
- Biological potency assays, such as receptor binding
assays
-
Download the course syllabus
 TISSUE CULTURE AND STAINING
(BioSc 260, summer quarter)
This is an introductory course in the theory and
concepts of animal cell and tissue culturing. Students
will learn the fundamentals in tissue culture
techniques, subculturing and maintenance of cell lines.
The following laboratory procedures will be performed in
the lab portion of the class:
- Use of CO2 incubators, laminar flow hood and inverted
microscope
- Cell counting techniques; hemocytometer and coulter
counter
- Preparation of media for tissue culture
- Sterile techniques and safety procedures
- Initiation and maintenance of primary cell culture,
adherent and suspension cell lines
- Isolation of mouse tumor cells or fibroblasts and
maintenance in culture
- Cell viability testing
- Staining techniques used for various cell types
- Bioassays and toxicology testing
- Genetic manipulation of tissue culture cells through
transfection
-
Download the course syllabus
 SKILL UPGRADE SHORT COURSES
This ongoing training program helps technicians and
research assistants expand and update their skill base
and enhance their employability. The courses were
developed by a consortium of biotech and biomedical
industry representatives and are taught by industry
experts in the evenings or on weekends.
- Flow Cytometry
- High Performance Liquid
Chromatography
- Applied Math for
Biotechnology
- Cloning and Expression
- Plant and Animal Tissue
Culture
- Introduction to
Bioinformatics
- Regulatory Affairs
-
Download the Biotech Upgrade Training Program Brochure
- Register for Short Courses
online or
call 206-533-6700.
|